Whitepapers/App Notes/Posters

Application Note: Anthocyanin purification from blackcurrant with SCPC-100

Posted: 9 January 2015 |

The blackcurrant is the edible berry of a shrub, which usually grows to 1-2 meters and can be found in Northern/Central Europe and Asia.

Anthocyanins are found in high concentrations in the fruit and give its characteristic dark purple color. Delphinidin 3-O-glucoside, delphinidin 3-Orutoside, cyanidin 3-O-glucoside and cyanidin 3-0-rutoside make up more than 97% of anthocyanin in the plant1. The crude extract is a complex matrix and the use of Centrifugal Partition Chromatography (CPC) enables purification of the targeted compound. 500mg of powder extract of blackcurrant was injected by CPC to purify anthocyanin.

Apparatus

An Armen SCPC-100 with 100mL column capacity was connected and controlled via an Armen Spot prep II system equipped with 50ml/mn quaternary gradient pump, UV/Vis detector and fraction collector. AGCPC software was used…

This application note is restricted - login or subscribe free to access

EPR Issue 1 2024Thank you for visiting our website. To access this content in full you'll need to login. It's completely free to subscribe, and in less than a minute you can continue reading. If you've already subscribed, great - just login.

Why subscribe? Join our growing community of thousands of industry professionals and gain access to:

  • bi-monthly issues in print and/or digital format
  • case studies, whitepapers, webinars and industry-leading content
  • breaking news and features
  • our extensive online archive of thousands of articles and years of past issues
  • ...And it's all free!

Click here to Subscribe today Login here

 

Related organisations