- Cancer Biology & Biomarkers
- Chromatography & Mass Spectrometry
- Contract Research, Clinical Trials and Outsourcing
- Drug Discovery
- Drug Targets
- Flow Cytometry
- Informatics & Lab Automation
- Ingredients, Excipients and Dosages
- Microbiology & RMMs
- NIR, PAT & QbD
- Raman Spectroscopy
- Screening, Assays & High-Content Analysis
- Thermal Processing
- Events & Workshops
Application Note: MALDI-ToF Mass Spectrometry – A valid alternative for microbial identification
11 January 2016 • Author(s): Wickham Laboratories Limited
Rapid and accurate identification of pathogenic microorganisms in areas such as pharmaceutical and medical device manufacturing are of paramount importance. Traditional identification techniques often require subcultures on selective medium, colony isolation, Gram staining or biochemical testing, which are labour intensive and time consuming.
Rapid microbiological methods (RMMs) for microbial identification have been widely adopted by the clinical microbiology environment due to their speed and accuracy resulting in reduced risk and improved patient safety. Furthermore, as RMMs generally reduce product release cycle time, labour and cost alongside supporting real-time data analysis and high throughput, it is unsurprising that these technologies are also being adopted by the pharmaceutical and medical device industries.
Matrix Assisted Light Desorption Ionisation Time-Of Flight (MALDI-ToF)
MALDI-ToF mass spectrometry, a phenotypical RMM for microbial identification, is a unique, highly accurate alternative method that facilitates cost-effective, precise and rapid data acquisition. Since its introduction by Karas and co-workers in 1987, MALDI-ToF mass spectrometry has been substantially developed and is now considered to be a real alternative for bacterial identification due to the provision of rapid and specific determination analogous to molecular sequencing techniques, with the benefit of significant time and cost savings.
ABB Analytical Measurement ACD/Labs ADInstruments Ltd Advanced Analytical Technologies GmbH Analytik Jena AG Astell Scientific Ltd Bachem AG Bibby Scientific Limited Bio-Rad Laboratories BioNavis Ltd Biopharma Group Black Swan Analysis Limited Charles Ischi AG | Kraemer Elektronik Cherwell Laboratories CI Precision Cobalt Light Systems Coulter Partners CPC Biotech srl Dassault Systèmes BIOVIA DiscoverX Edinburgh Instruments Enterprise System Partners (ESP) EUROGENTEC F.P.S. Food and Pharma Systems Srl IDBS JEOL Europe L.B. Bohle Maschinen + Verfahren GmbH Lab M Ltd. LabWare Linkam Scientific Instruments Limited Molins Technologies Multicore Dynamics Ltd Nanosurf New England Biolabs, Inc. Panasonic Biomedical Sales Europe B.V. PerkinElmer Inc ReAgent Russell Finex Limited Source BioScience Takara Clontech Tornado Spectral Systems Tuttnauer Watson-Marlow Fluid Technology Group Wickham Laboratories Limited Xylem Analytics YMC Europe GmbH Yusen Logistics