- Cancer Biology & Biomarkers
- Chromatography & Mass Spectrometry
- Contract Research, Clinical Trials and Outsourcing
- Drug Discovery
- Drug Targets
- Flow Cytometry
- Informatics & Lab Automation
- Ingredients, Excipients and Dosages
- Microbiology & RMMs
- NIR, PAT & QbD
- Raman Spectroscopy
- Screening, Assays & High-Content Analysis
- Thermal Processing
- Events & Workshops
Vitamin D Level Determination in Serum
12 February 2016 • Author: YMC Europe GmbH
Vitamin D3 (cholecalciferol) is essential for the human body. Low levels of vitamin D3 increase the risk of illness from a range of disorders. Babies, young children, pregnant mothers and old people are at a higher risk.
Traditionally, vitamin D deficiency has been associated with rickets, a disease in which the bone tissue does not properly form, leading to soft bones and skeletal deformities. However, research is revealing the importance of vitamin D in protecting against a host of other health problems.
What to Detect in Serum
The serum level of vitamin D is monitored to exclude deficiency symptoms. As vitamin D products and nutritional supplements often contain vitamin D3 and/or vitamin D2 (ergocalciferol), usually the concentration of 25-hydroxy vitamin D3 (25-OH-D3, calcidiol) is determined. 25-OH-vitamin D3 is the liver-generated storage form of vitamin D and provides a differentiated and meaningful impression of a patient’s vitamin D level.
As vitamin D2, D3 and 25-OH-vitamin D3 are structurally similar substances, the (U)HPLC separation is therefore very challenging. Often, special columns in combination with complex eluent mixtures have to be used. Using YMC-Triart C18 ExRS makes the separation much easier!
Baseline Separation in less than 6 min
The use of an UHPLC YMC-Triart C18 ExRS column under isocratic conditions allows a baseline separation of these three substances in less than 6 min. THF/n-hexane (10/90) was used as mobile phase with a flow rate of 0.65 mL/min.
ABB Analytical Measurement ACD/Labs ADInstruments Ltd Advanced Analytical Technologies GmbH Analytik Jena AG Astell Scientific Ltd B&W Tek Bachem AG Bibby Scientific Limited Bio-Rad Laboratories BioNavis Ltd Biopharma Group Black Swan Analysis Limited CAPSUGEL NV Charles Ischi AG | Kraemer Elektronik Cherwell Laboratories CI Precision Cobalt Light Systems Coulter Partners CPC Biotech srl Dassault Systèmes BIOVIA DiscoverX Edinburgh Instruments Enterprise System Partners (ESP) Eurofins BioPharma Product Testing EUROGENTEC F.P.S. Food and Pharma Systems Srl GE Analytical Instruments IDBS JEOL Europe L.B. Bohle Maschinen + Verfahren GmbH Lab M Ltd. LabWare Linkam Scientific Instruments Limited MA Business Metrohm Molins Technologies Multicore Dynamics Ltd Nanosurf New England Biolabs, Inc. Ocean Optics Panasonic Biomedical Sales Europe B.V. PerkinElmer Inc ReAgent Russell Finex Limited Source BioScience Takara Clontech Tornado Spectral Systems Tuttnauer Viavi Solutions, Inc Watson-Marlow Fluid Technology Group Wickham Laboratories Limited Xylem Analytics YMC Europe GmbH Yusen Logistics