Analysis of microRNA expression by qPCR

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publication date: Nov 23, 2007

Alteration of microRNA (miRNA) expression in a disease compared to a healthy state and/or correlation of miRNA expression with clinical parameters (like disease progression or therapy response), may indicate that miRNAs can serve as clinically relevant biomarkers1-3. An important first step for further functional characterisation is the information about differential miRNA expression in cellular processes such as; differentiation4,5, proliferation or apoptosis,6 that may determine which disease causing genes are specifically regulated by miRNAs, or vice versa; which genes regulate miRNA expression.

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