Lab-on-a-chip - Articles and news items

Microfluidics in cell-to-cell signalling measurements

Microfluidics in cell-to-cell signalling measurements

Drug Targets, Issue 3 2016 / 30 June 2016 / Laura D. Casto and Christopher A. Baker, University of Tennessee

Hormones are secreted molecules that carry biological information between cells. This type of cell-to-cell communication is vital to human life, and its dysregulation often underlies disease. In fact, the largest known class of cell surface receptors involved in hormone communication, G-protein coupled receptors, are also the largest class of known drug targets. Understanding these communication processes and their dysregulation requires an ability to ‘listen in’ on hormone communications with high fidelity. To do this, highly sensitive and specific measurements of often challenging-to-detect chemical species – for example, peptides and proteins – must be made from within complex matrices such as biological fluids and tissues. This challenge has given rise to sophisticated analytical techniques for in vivo hormone measurements, including implanted microelectrodes for in situ detection of electrochemically active hormones from model organisms, and microdialysis for in situ sampling of electrically inactive molecules for ex vivo measurements…

Lab-on-a-chip cell culture for metabolomics

Microfluidic chips: Lab-on-a-chip cell culture for metabolomics

Issue 3 2015 / 3 July 2015 / Laura A. Filla and James L. Edwards, Saint Louis University

Microfluidic devices (MFDs) have recently become popular as cell culture platforms for metabolomics due to reduced reagent requirements and the feasibility of flow-based studies. Such devices have the potential to transform pharmaceutical research by providing an online approach to high-throughput screening which can be coupled with a variety of analysis strategies. This brief review will focus on advantages and challenges of cell culture in microfluidic chips for use in targeted and untargeted metabolomics experiments. Additionally, advances in on-chip sample preparation methods, including solid phase extraction and lysing, will be explored…


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