Phenotypic drug discovery (PDD) - Articles and news items

Application Note: A phenotypic assay for metastasis

Application Note: A phenotypic assay for metastasis

Whitepapers / 8 January 2016 / BioTek

BioTek demonstrate a method for the generation of 3D spheroidal structures using a co-culture of a human breast cancer cell line and human fibroblasts embedded in an invasion matrix containing CXCL12 as chemoattractant…

Studying Cell Behaviour using a Phenotypic Approach

Video Interview: Studying Cell Behaviour using a Phenotypic Approach

Videos / 28 July 2013 / PerkinElmer Inc.

In this video interview, Dr. Chris Bakal from the Institute of Cancer Research, London, describes how his research group studies the shape changes of metastatic cells and how they spread through the body to cause disease. He explains how scientists perform high throughput RNA interference screens using the Opera® High Content Screening System, imaging millions of cells and quantifying individual shapes using a phenotypic approach…

High content imaging subpopulation analysis for phenotypic drug discovery

Issue 3 2008, Past issues / 19 June 2008 /

Phenotypic drug discovery (PDD) has come of age – again. Using a microscope to observe a cell, one of the oldest techniques available to a cellular biologist dates back to the 17th century studies of Antony van Leeuwenhoek and his characterisation of ‘animalcules’. These early analyses, which simply described the appearance of a cell or group of cells, are the basis for today’s phenotypic assays. Although this early work might seem irrelevant when compared to the powerful array of tools that modern science brings to bear on a problem, the use of cellular phenotype as a method of scientific investigation evolved over time into the primary method of drug discovery up through the 1970s. Cellular phenotypes and the phenotypic changes induced upon compound treatment were commonly followed in both basic science and drug discovery[1-3] and even led to the development of early forms of automated data acquisition and analysis[4].


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