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Protein-protein interactions (PPI) - Articles and news items

Duolink-Technology-for-HCS-Imagev2

Whitepaper: Duolink® Technology – Illuminating Insight into Protein Interactions by HCS

Whitepapers / 8 April 2014 / Sigma-Aldrich

Alongside established drug target classes, innovative approaches are addressing previously undruggable target classes such as protein–protein interactions.

Figure 1: Principle of protein fragment complementation. Two interacting proteins are fused to N and C terminal fragments of a fluorescent or luminescent reporter protein (generic depicted in yellow). Upon interaction, the activity of the reporter is reconstituted and can be read out as fluorescence upon excitation (2) or as luminescence upon substrate addition (3)

Cell-based assays for protein-protein interactions

Issue 5 2013, Screening / 22 October 2013 / Mark Wade, Center for Genomic Science of IIT@SEMM

Protein-protein interactions (PPI) form the backbone of all cellular signalling networks, and aberrant PPI contribute to the pathology of several diseases. Thus, strategies to identify PPI modulators are expected to be therapeutically beneficial. However, there are very few examples of clinically approved PPI modulators, reflecting the difficulties of identifying effective compounds for this target class. This perspective reviews the challenges associated with targeting PPI, and summarises the major strategies used to detect and disrupt PPI, with a particular focus on cell-based assays for PPI.

 

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