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Rapid Methods - Articles and news items

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Wickham Laboratories announces upcoming webinar on Rapid Methods

Supplier news / 7 August 2015 / Wickham Laboratories Limited

Join us for a discussion on Rapid Screening Techniques, October 1 3PM London/10AM New York…

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Rapid micro methods and the next generation in ATP bioluminescence

Issue 5 2012, Microbiology / RMMs / 25 October 2012 / Michael J. Miller, President, Microbiology Consultants, LLC and Noe Miyashita, Researcher, Hitachi Plant Technologies, Ltd

This is the fifth paper in our continuing series on Rapid Microbiological Methods (RMM) that will appear in European Pharmaceutical Review during 2012. As many of you know, I am always on the lookout for the next generation of rapid microbiological method (RMM) technologies and solutions. In this article, I have invited Noe Miyashita, a researcher from Hitachi Plant Technologies, to describe a novel ATP bioluminescence technology platform that she and her colleagues are currently working on. But in order to frame this discussion, it is appropriate to provide some background material on the fundamental basics of ATP bioluminescent methods.

ATP bioluminescence is the generation of light by a biological process, and is most recognised in the tails of the American firefly Photinus pyralis. First discovered in 1947 by William McElroy, he described the ATP bioluminescence reaction in which ATP (Adenosine Triphosphate) is enzymatically consumed to produce light. Specifically, in the presence of the substrate luciferin, the enzyme luciferase will use the energy from ATP to oxidise luciferin and release photons (light at a wavelength of 562 nanometres). The photons can then be detected and measured by a luminometer equipped with a photomultiplier tube. Figure 1 provides an illustration of this chemical reaction.

Rapid Methods Supplement 2011

Rapid Methods supplement 2011

Issue 5 2011, Microbiology / RMMs, Supplements / 24 October 2011 / Bryan S. Riley, Michael J. Miller, Youwen Pan

A regulators view.
Microbiology series: Nucleic acid and gene amplification-based technologies.
Challenges and strategies for application in the pharmaceutical industry.

 

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