Whitepaper: Analysis of a Mammalian cell culture

In this free whitepaper we discuss real-time, in situ bioprocess understanding in accordance with the PAT initiative and comparison of Raman and NIR spectra of cell culture components…

Key Issues

• Real-time, in situ bioprocess understanding in accordance with the PAT initiative
• Comparison of Raman and NIR spectra of cell culture components
• Quantitative determination of mammalian cell culture nutrients and metabolites Introduction 

Mammalian cell cultures are complex processes by which cells are grown under controlled conditions. In industry, mammalian cell cultures are fundamental to the manufacture of viral vaccines and other biologic products. In the 1980s Chinese hamster ovary (CHO) cells began to revolutionize mass culture for medicines, and they continue to remain the industry‘s workhorse for therapeutic protein production.

The work described here involves the development and application of new bioprocess analytics in accordance with the aims of “Quality by Design” (QbD) and the U.S. FDA’s Process Analytical Technology (PAT) initiative. Current research is focused on gaining a better understanding of mammalian cell cultures via cultivation of CHO cells for therapeutic protein production. Understanding and monitoring the stages of this bioprocess is vital to ensure healthy progression of the cell culture.