Implementing reporter assay-based MAT for rapid pyrogen detection
Sponsored by 
FUJIFILM Wako Chemicals Europe GmbH2026-09-02T14:00:002026-09-02T14:00:002026-09-02T15:00:00
Watch this webinar to discover how reporter assay-based MAT can help laboratories accelerate pyrogen testing, simplify workflows and support the transition to animal-free testing while maintaining confidence in results.
The webinar featured expert insights into how the addition of supplements can improve the NEP spike recovery in previously hard-to-test pharmaceuticals. Validation data was presented that illustrated the use in actual pharmaceutical products, as well as the practical benefits of adding supplements to enhance assay performance and confirming the method’s applicability across diverse pharmaceutical formulations.
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What you will learn:
- Learn about the development of a new MAT method using a luciferase assay as an accurate and rapid alternative to RPT
- Discover the benefits of reporter assay based-MAT, including how it can significantly reduce testing time and simplify operation, leading to decreased variability in results between testers, and how using a cell line instead of PBMCs ensures lot-to-lot variation and stable supply.
- Understand how to perform primary validation and method suitability tests in accordance with Ph. Eur. 2.6.30.
- Explore the case studies: improving NEP spike recovery with supplement addition and high throughput testing using 384-well plates
Whether you are evaluating alternative pyrogen testing methods or looking to improve laboratory efficiency, this webinar provided practical insights you can apply to your own testing workflows.