whitepaper

Application note: Comprehensive fractionation of herbal medicine components by PDA-ELSD-triggered preparative LC

This article introduces a comprehensive fractionation method for both UV absorbing and non-UV absorbing compounds.

Preparative LC is widely known as a means of purifying target compounds from mixtures. Ultraviolet (UV) or photodiode array (PDA) detectors are commonly used in ordinary preparative LC. In this case, preparative purification of all non-UV absorbing analytes is not possible.

This article introduces a comprehensive fractionation method for both UV absorbing and non-UV absorbing compounds using a newly designed PDAELSD- triggered preparative LC system equipped with an evaporative light scattering detector (ELSD) in addition to the PDA.

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