Application note

Application note: Validating recombinant cascade reagents in 3 simple steps

Endotoxin testing continues to evolve alongside the industry’s need for more sustainable methods that reduce ecological impact. Nicola Reid, Associate Director of Endotoxin Products, Charles River Labs, reflects on these developments which are driven by the 3Rs (replacement, reduction and refinement).

Although current practices responsibly source and utilize horseshoe crabs, there remains a significant opportunity for innovation with alternative testing methods. The advent of recombinant cascade reagents (rCR) represents a transformative approach, utilizing biotechnology to replicate the natural endotoxin detection capabilities of horseshoe crabs. This shift aligns with broader sustainability goals in the industry.