Jacobsen NW, Nielsen LD. Two-dimensional liquid chromatography (2D-LC) with multiple heart-cutting in a troubleshooting laboratory. European Pharmaceutical Review. 2017;3:60-63



  1. Stoll DR, Li X, Wang X, Carr PW, Porter SEG, Rutan SC. Fast, comprehensive two-dimensional liquid chromatography, J Chromatogr A. 2007;1168(1–2):3-43. doi:10.1016/j.chroma.2007.08.054.
  2. Pursch M, Buckenmaier S. Loop-based multiple heart-cutting two-dimensional liquid chromatography for target analysis in complex matrices. Anal Chem. 2015;87(10):5310-7. doi: 10.1021/acs.analchem.5b00492.
  3. Chen M, Wang L, Dong H, Shao X, Wu D, Liu B, Zhang X, Chen C. Quantitative method for analysis of tobacco-specific N-nitrosamines in mainstream cigarette smoke by using heart-cutting two-dimensional liquid chromatography with tandem mass spectrometry. J Sep Sci. 2017;40(9):1920-1927. doi: 10.1002/jssc.201601367.
  4. van de Schans MGM, Blokland MH, Zoontjes PW, Mulder PPJ, Nielen MWF. Multiple heart-cutting two dimensional liquid chromatography quadrupole time-of-flight mass spectrometry of pyrrolizidine alkaloids. J Chromatogr A. 2017;1503:38-48. doi: 10.1016/j.chroma.2017.04.059.
  5. Qiao X, Wang Q, Song W, Qian Y, Xiao Y, An R, Guo DA, Ye M. A chemical profiling solution for Chinese medicine formulas using comprehensive and loop-based multiple heart-cutting two-dimensional liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. J Chromatogr A. 2016;1438:198-204. doi: 10.1016/j.chroma.2016.02.034.
  6. Shackman JG, Kleintop BL. Peak purity assessment in a triple-active fixed-dose combination drug product related substances method using a commercial two-dimensional liquid chromatography system. J Sep Sci. 2014;37(19):2688-95. doi: 10.1002/jssc.201400515.
  7. Barhate CL, Regalado EL, Contrella ND, Lee J, Jo J, Makarov AA, Armstrong DW, Welch CJ. Ultrafast Chiral Chromatography as the Second Dimension in Two-Dimensional Liquid Chromatography Experiments. Anal Chem. 2017;89(6):3545-3553. doi: 10.1021/acs.analchem.6b04834.
  8. Sandra K, Vanhoenacker G, Vandenheede I, Steenbeke M, Joseph M, Sandra P. Multiple heart-cutting and comprehensive two-dimensional liquid chromatography hyphenated to mass spectrometry for the characterization of the antibody-drug conjugate ado-trastuzumab emtansine. J Chromatogr B Analyt Technol Biomed Life Sci. 2016;1032:119-30. doi: 10.1016/j.jchromb.2016.04.040.
  9. Petersson P, Haselmann K, Buckenmaier S. Multiple heart-cutting two dimensional liquid chromatography mass spectrometry: Towards real time determination of related impurities of bio-pharmaceuticals in salt based separation methods. J Chromatogr A. 2016;1468:95-101. doi: 10.1016/j.chroma.2016.09.023.
  10. Luo H, Zhong W, Yang J, Zhuang P, Meng F, Caldwell J, Mao B, Welch CJ. 2D-LC as an on-line desalting tool allowing peptide identification directly from MS unfriendly HPLC methods. 5. J Pharm Biomed Anal. 2017;137:139-145. doi: 10.1016/j.jpba.2016.11.012.
  11. Yang SH, Wang J, Zhang K. Validation of a two-dimensional liquid chromatography method for quality control testing of pharmaceutical materials. J Chromatogr A. 2017;1492:89-97. doi: 10.1016/j.chroma.2017.02.074.


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