Whitepapers/App Notes/Posters

Whitepaper: Light scattering for determination of molecular weight and radius of gyration

Posted: 25 June 2019 | | No comments yet

Light Scattering of macromolecules in solution has seen increasing interest over the past decades from scientists studying polymers and proteins.

Since the mid 1950s and its inception, size exclusion (or gel permeation) chromatography (GPC/ SEC) has been the standard technique for the characterisation of molecular weight distributions by separating macromolecules according to their size. The addition of light scattering detection online with GPC/SEC systems allows the measurement of true – or absolute – molecular weight of macromolecules, as opposed to the relative values obtained from conventional column calibration techniques.











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