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Electrospray Ionisation (ESI) Mass Spectrometry - Articles and news items

Mass Spectrometry In-depth focus

Mass Spectrometry: In-depth focus 2014

Chromatography, Issue 2 2014, Supplements / 15 April 2014 / Paul J. Gates, Ioannis Papayannopoulos

In this free-to-view in-depth focus: Some tricks and tools for structural characterisation of natural products using Electrospray mass spectrometry, Mass spectrometry in the biopharmaceutical industry: from the mundane to the cutting edge, and Mass Spectrometry roundtable…

Birthe V. Nielsen, Faculty of Engineering and Science, University of Greenwich

Practical considerations in analysing biologically active peptides by Electrospray Ionisation (ESI) Mass Spectrometry

Chromatography, Issue 5 2013 / 22 October 2013 / Birthe V. Nielsen, Faculty of Engineering and Science, University of Greenwich

Neuromodulators such as calcitonin gene-related peptide (CGRP) and vasoactive intestinal peptide (VIP) act as biomarkers for pain assessment (pre-clinical). These markers can be detected at low concentrations by Electrospray Ionisation (ESI) Mass Spectrometry (MS). Currently, little is known about the factors affecting responsiveness in the ESI process though the response of peptide ions depends heavily on analyte basicity in solution, pKb, sampling and instrumental conditions. Other factors may be equally important, however, not enough is known about the link between peptide characteristics and selectivity in the ESI process. Here, we review our findings on these links to improve the analyte signal and allow robust quantitation of these important peptide biomarkers…

 

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