Application note: Benchmarking the Microsart^® AMP Mycoplasma kit against products from two other suppliers

This application note compares Sartorius’ Microsart AMP Mycoplasma kit with two other mycoplasma detection kits that are also based on DNA isolation with subsequent real-time PCR analysis.

Among the world’s smallest bacteria, mycoplasmas are capable of independent reproduction. They belong to the class of mollicutes and have a very slow parasitic growth. The contamination of cell cultures remains a major problem. An array of physiological and biochemical parameters are affected by the presence of mycoplasma in cells cultures. The infection causes changes in metabolism, growth, viability, macromolecule synthesis, morphology, etc, and therefore a sensitive routine testing for contamination of cell cultures is essential.