- Cancer Biology & Biomarkers
- Chromatography & Mass Spectrometry
- Contract Research, Clinical Trials and Outsourcing
- Drug Discovery
- Drug Targets
- Flow Cytometry
- Informatics & Lab Automation
- Ingredients, Excipients and Dosages
- Microbiology & RMMs
- NIR, PAT & QbD
- Raman Spectroscopy
- Screening, Assays & High-Content Analysis
- Thermal Processing
- Events & Workshops
Whitepaper: Maintaining sample integrity with aerosol-blocking tips
7 October 2016 • Author(s): Thermo Fisher Scientific
Thermo Scientific™ ART™ self-sealing barrier tips were tested by an independent laboratory to demonstrate their ability to preserve sample integrity during use in PCR. First, the ability of the proprietary ART barrier to prevent contamination was tested and compared to other filter tips.
Second, the ability to recover liquid which was overdrawn into the ART barrier was determined. Third, the ART barrier was tested to show that it does not compromise or inhibit PCR if accidentally contacted by samples used in PCR.
Preserving the integrity of PCR conditions and samples is essential. For this reason, Thermo Fisher Scientific developed ART self-sealing barrier tips to prevent contamination and provide reproducible sample delivery.
It is important to select an aerosol-blocking tip based on its ability to prevent aerosols and liquids from contaminating the pipettor or subsequent samples. A total of three brands of tips were used in this test, but only ART self-sealing barrier tips were able to prevent contamination.
In the event the sample contacts the filter of a tip, it may be necessary to retrieve the sample. To prove the ability of the ART barrier tip to minimize sample holdback, retention was quantified using water.
Because of the sensitivity of PCR, a variety of factors can compromise the integrity of the reaction. This illustrates the importance of using aerosol-blocking tips which do not inhibit PCR or otherwise introduce contaminants into precious samples. To demonstrate that ART self-sealing barrier tips do not inhibit PCR, an independent laboratory evaluated ART barrier tips using protocols that duplicated actual laboratory conditions.
ABB Analytical Measurement ACD/Labs ADInstruments Ltd Advanced Analytical Technologies GmbH Analytik Jena AG Astell Scientific Ltd B&W Tek Bachem AG Bibby Scientific Limited Bio-Rad Laboratories BioNavis Ltd Biopharma Group Black Swan Analysis Limited CAPSUGEL NV Charles Ischi AG | Kraemer Elektronik Cherwell Laboratories CI Precision Cobalt Light Systems Coulter Partners CPC Biotech srl Dassault Systèmes BIOVIA DiscoverX Edinburgh Instruments Enterprise System Partners (ESP) Eurofins BioPharma Product Testing EUROGENTEC F.P.S. Food and Pharma Systems Srl GE Analytical Instruments IDBS JEOL Europe L.B. Bohle Maschinen + Verfahren GmbH Lab M Ltd. LabWare Linkam Scientific Instruments Limited MA Business Metrohm Molins Technologies Multicore Dynamics Ltd Nanosurf New England Biolabs, Inc. Ocean Optics Panasonic Biomedical Sales Europe B.V. PerkinElmer Inc ReAgent Russell Finex Limited Source BioScience Takara Clontech Tornado Spectral Systems Tuttnauer Viavi Solutions, Inc Watson-Marlow Fluid Technology Group Wickham Laboratories Limited Xylem Analytics YMC Europe GmbH Yusen Logistics