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LC-MS In-Depth Focus 2015

Posted: 20 April 2015 |

In this free-to-view LC-MS in-depth focus, we delve into the world of glycans, and how their natural complexity necessitates sophisticated sequencing techniques, while another article aims to demonstrate the versatility of LC-MS and the ease with which it can be combined with a variety of MS systems, focusing particularly on nanoLC…

LC-MS In-Depth Focus 2015
  • HPLC mass spectral analysis of glycans
    David Elder, Phil Borman and George Okafo, GSK & William McDowell, PolyTherics Ltd
    The terms glycan and oligosaccharide are defined by the International Union of Pure and Applied Chemistry (IUPAC) as ‘compounds consisting of a large number of monosaccharides linked glycosidically’. However, in common usage the term glycan has come to mean the carbohydrate section of a glycoconjugate, for example, a glycoprotein, glycolipid, or a proteoglycan, even if the carbohydrate is only an oligosaccharide. Glycans usually consist solely of O-glycosidic linkages of monosaccharides. For example, cellulose is a glycan composed of β-1,4-linked D-glucose, whereas chitin is a glycan composed of β-1,4-linked N-acetyl-D-glucosamine. Glycans can be either homo or heteropolymers of monosaccharide residues, and structurally they can be linear or branched in nature. Glycans can be further characterised as either N-linked (connected to the conjugate via a nitrogen atom in the sidechain of an amino acid like asparagine) or O-linked (where the bond is via the oxygen atom in serine or threonine residues)…
  • Analysis of limited and complex samples with nanoLC-MS
    Hanne Røberg-Larsen, University of Oslo
    Liquid chromatography-mass spectrometry (LC-MS) is a diverse technique used in analysis of substances such as biomolecules and environmental targets. The analytes are separated on a stationary phase inside an LC column, before they are separated based on their mass-to-charge ratio (m/z) in a mass spectrometer and detected. This technique has great potential in both qualitative and quantitative analysis using either accurate mass for identification, external standards for quantification or a combination of both. Today, these techniques can be used to identify biomarkers and environmental pollutants and to diagnose disease among other uses. This article aims to demonstrate just how versatile LC-MS is: how LC can be combined with a variety of MS systems and the increasing popularity of nanoLC…
  • LC-MS Roundtable
    Featuring: Kevin Ray PhD, Senior Manager, Analytical Research & Development, Sigma-Aldrich / Stéphane Moreau, Product manager MS range, Shimadzu Europa GmbH / Iain Gibb, Market Development Manager EMEA Pharmaceutical, Sciex / Sean M. McCarthy, Senior Scientific Operations Manager, Biopharmaceutical Business, Waters Corporation
    Moderated by Moderator David Elder, GlaxoSmithKline and JPAG…

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