Stathmin immobilisation on a chip using an oligo-cysteine tag
27 March 2007 | By Kazuyuki Nakamura, M.D., Ph.D., Professor and Chairman, Department of Biochemistry and Functional Proteomics, Yamaguchi University Graduate School of Medicine
Protein chip technology is essential for high through-put functional proteomics. In this review the development of a novel protein tag consisting of five tandem cysteine repeats (Cys-tag) at C-terminus of proteins which was covalently attached to the surface of a maleimide-modified diamond-like carbon-coated silicon chip substrate is described.