Optimising analytical separations of synthetic RNA with modified HPLC

Oligonucleotides are diverse in nature, with a wide range of applications. Here, Dr Anastassia Kanavarioti and Dr Sandra Rontree explore the challenges of oligonucleotide analysis in the life sciences sector and outline the different HPLC methods available for the job.

RNA strands, a type of oligonucleotide

OLIGONUCLEOTIDES are the starting point for many molecular biology and synthetic biology applications. Used in a wide variety of ways, from biopharmaceutical therapeutics to clinical diagnostics and forensic research, the purification and analysis of oligonucleotides is becoming increasingly important across the life sciences sector.1

During the synthesis of oligonucleotides, smaller side chains, failure sequences and impurities can become present, which, depending on the downstream application, can negatively impact results. When choosing a technique to purify and characterise oligonucleotides, the type of solution being eluted and the goals of the analysis are both important. For example, if the downstream application requires only the full-length sequence, using a purification technique to create a purer oligonucleotide, such as high-performance liquid chromatography (HPLC), will be crucial.

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