Whitepapers/App Notes/Posters

Application note: Solving the aggregation problem of HEK 293 cells

Posted: 15 December 2021 | | No comments yet

In this application note, we show a successful example of the adaptation of a human recombinant protein-expressing HEK293 cell line.

In this application note, we show a successful example of the adaptation of a human recombinant protein-expressing HEK293 cell line from serum-supplemented attachment culture to serum-free single cell suspension culture using the New Brunswick S41i CO2 incubator shaker. We expect that such a cell line can be used in bioprocess applications using the typical batch or fed-batch methods established for conventional CHO cell culture in stirred tank bioreactors.












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