Application note: Miniaturised multi-piece DNA assembly
The Echo Liquid Handler has demonstrated the ability to generate DNA constructs using various assembly chemistries with a miniaturised protocol.
Interchanging smaller, modular pieces of DNA is the preferred method for many DNA construction workflows due to decreased synthesis cost and faster progression along the design, build, test, and learn cycle. In this app note, the NEB NEBuilder HiFi DNA Assembly Cloning Kit was combined with the Echo 525 Liquid Handler to assemble a construct over five modular pieces. The reaction was miniaturized 40x from the manufacturer recommended volume, representing a significant savings in reagent costs.
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