Whitepapers/App Notes/Posters

Application note: Improved RP-HPLC to analyse intact mAb’s

Posted: 11 March 2021 | | No comments yet

A fast, high-resolution, and reproducible reverse phase HPLC method using the BIOshell™ A400 Protein C4 column to analyse the intact mAb Trastuzumab.

This application note demonstrates that the BIOshell™ A400 Protein C4 column, based on a wide-pore (400 Å) Fused-Core® particle design, is ideally suited for separating the intact therapeutic monoclonal antibody (mAb) Trastuzumab. The retention time, area precision, quantification, and robustness make this RP-HPLC method ideal for biopharma QC. The method can also be used to monitor degraded product created by heat stress, making it suitable for stability studies.