Whitepaper/App Note

Poster: Optimised purity and recovery of a monoclonal antibody using mixed-mode chromatography media

Posted: 30 June 2017 | | No comments yet

Bio-Rad discuss the technique of optimising the purity of a monoclonal antibody using mixed-mode chromatography media…

Purity, recovery, and elution volume are important factors and parameters when screening multiple media (resins) for the development of efficient, robust, and economical production processes. Among a selection of mixed-mode media evaluated for the purification of mAb S, CHT™ Ceramic Hydroxyapatite provided the best monomer recovery at 83% in the smallest elution volume with a target purity of 99.5%. Using a bind and elute strategy provided enhanced purification power compared to flow-through modes.

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