Whitepaper: Making MS-based glycan analysis easier
Recent technological advances have made it easier to cope with the complexity of mass spectrometry (MS) based glycan analysis.
Glycosylation occurs in expression systems for producing biological drugs, creating a complex spectrum of N- and O-linked glycans that vary significantly with regards to in vivo stability, solubility, activity, safety, and efficacy. This makes glycoprotein analysis a challenging task that is performed at several levels: intact protein, subunit, peptide, and glycan. This white paper presents recent advances in MS-based methods to reduce the task’s complexity at all four levels.