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Issue 3 2015



PCR In-Depth Focus 2015

3 July 2015 | By

In this PCR in-depth focus: Mikael Kubista from TATAA Biocenter addresses biological heterogeneity with single cell profiling, a look at quantitative PCR in the assessment of novel hepatic cell models, plus Q&A with Bio-Rad’s Javier Alba...


Microfluidic chips: Lab-on-a-chip cell culture for metabolomics

3 July 2015 | By Laura A. Filla and James L. Edwards, Saint Louis University

Microfluidic devices (MFDs) have recently become popular as cell culture platforms for metabolomics due to reduced reagent requirements and the feasibility of flow-based studies. Such devices have the potential to transform pharmaceutical research by providing an online approach to high-throughput screening which can be coupled with a variety of analysis…


Polyelectrolyte complexes as nanoparticulate drug delivery systems

3 July 2015 | By Anita Umerska, University of Anger and Lidia Tajber, Trinity College Dublin

Polyelectrolyte complexes at the nanoscale (polyelectrolyte complex nanoparticles; PECNs) can be harnessed as drug delivery systems for a range of low molecular weight drugs as well as peptides and proteins. The mild method of manufacture of PECNs results in the protection of the loaded bioactive and the choice of polyions…


Revolutionising Raman with the transmission technique

3 July 2015 | By Julien Villaumié and Hilary Jeffreys, Actavis

All regulatory agencies require that manufacturers accurately determine the amount of drug in a medicinal product so that the product can be deemed fit for patients. This forms part of batch release testing performed by Quality Control (QC) laboratories. The drug assay and/or content uniformity of the dosage units is…


Universal detection in high performance liquid chromatography

3 July 2015 | By David P. Elder, Phil Borman and George Okafo - GlaxoSmithKline

The most commonly used analytical technique for the testing of drug molecules is reversed-phase high performance liquid chromatography (RP-HPLC) with ultraviolet (UV)/visible detection. However, one of the biggest constraints affecting UV/visible detectors is their inability to generate adequate sensitivity for analytes lacking a suitable UV chromophore.


Fast quantitative 2D NMR for quantifying analytes in complex mixtures

3 July 2015 | By Patrick Giraudeau, University of Nantes

Nuclear magnetic resonance (NMR) spectrometry is a major quantitative tool in pharmaceutical analysis, owing to its extremely high accuracy. However, absolute quantification in complex mixtures is often hampered by spectral overlap which considerably reduces the quantitative performance of NMR.


PAT Series: Applicability of process analytical tools to bioprocessing trends

3 July 2015 | By Payal Roychoudhury, PhD, formerly AstraZeneca

The past decade has been a period of unparalleled change and development in the fermentation industry. As the nature of this industry evolves, and in particular, with the increasing prominence of the new biopharmaceuticals (therapeutic proteins, diagnostic enzymes and monoclonal antibodies) the need for effective bioprocess monitoring grows in importance1.