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Application note: Setup and transfer of a gradient HILIC method

Posted: 16 December 2021 | | No comments yet

A fast and reproducible gradient HILIC method was set up for the impurity analysis of the hydrophilic temozolomide.

The transfer of LC methods from one instrument to another is a recurring task in many analytical labs. For example, when method development is realised in a lab different than the lab for the final method implementation, such as outsourcing to contract labs.

The method transfer from an Agilent™ 1260 Infinity™ LC system to a Thermo Scientific™ Vanquish™ Core HPLC system was straightforward, providing equivalent results with slightly more repeatable retention times and narrower peaks.












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